Journal: Environmental Health Perspectives

Article Title: Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice

doi: 10.1289/EHP8428

Figure Lengend Snippet: Antibodies used for dot blot analyses.

Article Snippet: Amyloid oligomers (A11) , StressMarq , 1:1,000 , Rabbit , SPC-506D.

Techniques: Dot Blot

Journal: Molecular genetics and metabolism

Article Title: Glucocerebrosidase haploinsufficiency in A53T α-synuclein mice impacts disease onset and course

doi: 10.1016/j.ymgme.2017.11.001

Figure Lengend Snippet: (A) Representative images of immunofluorescence analysis depicting SNCA inclusion pathology in the pons of symptomatic SNCAA53T hemizygote (top two panels) or SNCAA53T homozygote (lower two panels) mice with gba+/+or gba+/− using anti-p62 antibody and Syn 506. Merged images counterstained with 4,6-diamidino-2-phenylindole (DAPI) are shown (right). (B) Quantitative analysis of SNCA burden in the pons using antibody Syn 506. (C) Representative images of immunofluorescence analysis depicting SNCA inclusion pathology in the midbrain of symptomatic SNCAA53T hemizygous or homozygous mice with gba+/+or gba+/− using anti-TH and Syn 506. (D) Immunoblotting of three proteins, p62, SNCA and TH, evaluated by immunofluorescence and their quantification (E, F, G), N = 3. (H) Immunohistochemical analysis depicting pathology in the hypothalamus, spinal cord, cerebellum and motor cortex in symptomatic SNCAA53T hemizygous or SNCAA53T homozygous mice with gba+/+or gba+/− using antibodies to pSer129 α-syn (EP1536Y or 81A). Scale bar 50 μm.

Article Snippet: Tissue sections were stained with: anti-pSer129 SNCA antibody 81A [ 26 ], anti-pSer129 SNCA antibody EP1536Y (Abcam), anti-SNCA antibody Syn 506 [ 27 , 28 ] anti-p62/sequestrome-1 (SQSTM1; Proteintech) (Chicago, IL) and anti-tyrosine hydroxylase antibody (TH; Millipore) (Fisher Scientific).

Techniques: Immunofluorescence, Western Blot, Immunohistochemical staining

Journal:

Article Title: Quantitative, Competitive PCR Analysis of Porcine Circovirus DNA in Serum from Pigs with Postweaning Multisystemic Wasting Syndrome

doi:

Figure Lengend Snippet: Construction of wild-type and competitor plasmids and validation of cPCR. (A) Diagrammatic representation of PCV2 wild-type and competitor constructs. The whole genome (1,768 nucleotides) of PCV2 was cloned with a SacII restriction enzyme into the pBKSII(+) vector. The largest two open reading frames (ORFs) in the PCV2 genome are indicated by open arrows. Two small filled arrows point out the primer binding sites. A portion of the GFP coding sequence as the insert for the construction of the competitor plasmid is demonstrated by a filled box. (B and C) Quantification of a known amount of PCV2 wild-type DNA. (B) cPCR assay performed with two series of 10-fold dilutions of competitor DNA (lanes 1 to 6, 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, and 1 pg, respectively; lanes 7 to 12, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, and 1 fg, respectively) to compete against two fixed amount of PCV2 wild-type DNA per reaction (lanes 1 to 6, 1 ng; lanes 7 to 12, 1 pg). Black bars above the lane numbers indicate the equivalent point. (C) The densities of DNA bands were measured with SPOT DENSO software by using an AlphaImager 2200 instrument, and values representing the average density per area unit were plotted. The numbers on the x axis correspond to the respective lane in panel B. The value determined for the wild-type DNA was in excellent agreement with the actual input amount.

Article Snippet: The equivalence points, at which the 502- and 506-bp bands and the 761- and 765-bp bands had equal intensities, were determined by visual inspection and densitometric scanning in an AlphaImager 2200 instrument (Alpha Innotech Corporation).

Techniques: Construct, Clone Assay, Plasmid Preparation, Binding Assay, Sequencing, Software

Journal: Nutrients

Article Title: Omega-3 Polyunsaturated Fatty Acids Inhibit the Function of Human URAT1, a Renal Urate Re-absorber

doi: 10.3390/nu12061601

Figure Lengend Snippet: Key resources.

Article Snippet: γ-Linolenic acid , Cayman Chemical , Cat# 90220; CAS: 506-26-3; Purity: ≥98%.

Techniques: Bicinchoninic Acid Protein Assay, Plasmid Preparation, Recombinant, Sequencing, Software